Biofilm forming Enterococci and their Status as Emerging Multidrug Resistant Bacteria

Urine specimens were collected aseptically and inoculated directly onto CLED MacConkey agar medium incubated overnight. Single target colonies sub cultured, identification was based on culture, morphology, biochemical characteristics. Enterococci isolates subjected to biofilm assay (Tube method), resistance pattern determined for both biofilm-formers non-biofilm-formers. Genomic DNA extracted using the Bioneer kit, gene virulence detected by PCR agarose gel electrophoresis. 148 Patients having one or more urinary symptoms study population. The aim of determine multidrug resistant ability biofilm-forming Enterococci. A number growths encountered; accounted only 9.46% (14), highest prevalence seen in age group 21-30 (35.71%), females (64.29%) prone enterococcal infection than their males (35.71) counterpart. There is a relationship between production antibiotic because isolate produced bands against esp with an average 510kbp. Biofilm forming strains showed gentamicin penicillin (83.3%), vancomycin can be considered good alternative therapy UTIs its lower (75%).